Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level
Identifieur interne : 004207 ( Main/Exploration ); précédent : 004206; suivant : 004208Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level
Auteurs : Warren Albertin [France] ; Aurélie Panfili [France] ; Cécile Miot-Sertier [France] ; Aurélie Goulielmakis [France] ; Adline Delcamp [France] ; Franck Salin [France] ; Aline Lonvaud-Funel [France] ; Chris Curtin [Australie] ; Isabelle Masneuf-Pomarede [France]Source :
- Food microbiology [ 0740-0020 ] ; 2014.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Vin.
English descriptors
- KwdEn :
Abstract
Although many yeasts are useful for food production and beverage, some species may cause spoilage with important economic loss. This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.
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uniqKey="Panfili A" first="Aurélie" last="Panfili">Aurélie Panfili</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Univ. de Bordeaux, ISW, EA 4577, Unité de recherche Œnologie</s1><s2>33140 Villenave d'Omon</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave d'Omon</settlement></placeName></affiliation></author><author><name sortKey="Miot Sertier, Cecile" sort="Miot Sertier, Cecile" uniqKey="Miot Sertier C" first="Cécile" last="Miot-Sertier">Cécile Miot-Sertier</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>INRA, ISW, USC 1219 OENOLOGIE</s1><s2>33140 Villenave d'Omon</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave d'Omon</settlement></placeName></affiliation></author><author><name sortKey="Goulielmakis, Aurelie" sort="Goulielmakis, Aurelie" uniqKey="Goulielmakis A" first="Aurélie" last="Goulielmakis">Aurélie Goulielmakis</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>CRB Œnologie, Centre de Ressources Biologiques Oenologique, ISW</s1><s2>33140 Villenave d'Omon</s2><s3>FRA</s3><sZ>4 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave d'Omon</settlement></placeName></affiliation></author><author><name sortKey="Delcamp, Adline" sort="Delcamp, Adline" uniqKey="Delcamp A" first="Adline" last="Delcamp">Adline Delcamp</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>INRA, UMR Biodiversité Genes et Ecosystèmes, PlateForme Génomique</s1><s2>33600 Cestas</s2><s3>FRA</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Cestas</settlement></placeName></affiliation></author><author><name sortKey="Salin, Franck" sort="Salin, Franck" uniqKey="Salin F" first="Franck" last="Salin">Franck Salin</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>INRA, UMR Biodiversité Genes et Ecosystèmes, PlateForme Génomique</s1><s2>33600 Cestas</s2><s3>FRA</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Cestas</settlement></placeName></affiliation></author><author><name sortKey="Lonvaud Funel, Aline" sort="Lonvaud Funel, Aline" uniqKey="Lonvaud Funel A" first="Aline" last="Lonvaud-Funel">Aline Lonvaud-Funel</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Univ. de Bordeaux, ISW, EA 4577, Unité de recherche Œnologie</s1><s2>33140 Villenave d'Omon</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave d'Omon</settlement></placeName></affiliation></author><author><name sortKey="Curtin, Chris" sort="Curtin, Chris" uniqKey="Curtin C" first="Chris" last="Curtin">Chris Curtin</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>The Australian Wine Research Institute</s1><s2>Glen Osmond, Adelaide, SA</s2><s3>AUS</s3><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>The Australian Wine Research Institute</wicri:noRegion></affiliation></author><author><name sortKey="Masneuf Pomarede, Isabelle" sort="Masneuf Pomarede, Isabelle" uniqKey="Masneuf Pomarede I" first="Isabelle" last="Masneuf-Pomarede">Isabelle Masneuf-Pomarede</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. de Bordeaux, ISW, EA 4577, Unité de recherche Œnologie</s1><s2>33140 Villenave d'Omon</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>33140 Villenave d'Omon</wicri:noRegion><wicri:noRegion>Unité de recherche Œnologie</wicri:noRegion><wicri:noRegion>33140 Villenave d'Omon</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Bordeaux Sciences Agro</s1><s2>33175 Gradignan</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>33175 Gradignan</wicri:noRegion><wicri:noRegion>Bordeaux Sciences Agro</wicri:noRegion><wicri:noRegion>Bordeaux Sciences Agro</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Food microbiology</title><title level="j" type="abbreviated">Food microbiol.</title><idno type="ISSN">0740-0020</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Food microbiology</title><title level="j" type="abbreviated">Food microbiol.</title><idno type="ISSN">0740-0020</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Brettanomyces</term><term>Development</term><term>Genotype</term><term>Marker</term><term>Strain</term><term>Wine</term><term>Yeast</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Développement</term><term>Marqueur</term><term>Génotype</term><term>Brettanomyces</term><term>Souche</term><term>Levure</term><term>Vin</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although many yeasts are useful for food production and beverage, some species may cause spoilage with important economic loss. This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Cestas</li><li>Gradignan</li><li>Villenave d'Omon</li></settlement></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Albertin, Warren" sort="Albertin, Warren" uniqKey="Albertin W" first="Warren" last="Albertin">Warren Albertin</name></region><name sortKey="Albertin, Warren" sort="Albertin, Warren" uniqKey="Albertin W" first="Warren" last="Albertin">Warren Albertin</name><name sortKey="Delcamp, Adline" sort="Delcamp, Adline" uniqKey="Delcamp A" first="Adline" last="Delcamp">Adline Delcamp</name><name sortKey="Goulielmakis, Aurelie" sort="Goulielmakis, Aurelie" uniqKey="Goulielmakis A" first="Aurélie" last="Goulielmakis">Aurélie Goulielmakis</name><name sortKey="Lonvaud Funel, Aline" sort="Lonvaud Funel, Aline" uniqKey="Lonvaud Funel A" first="Aline" last="Lonvaud-Funel">Aline Lonvaud-Funel</name><name sortKey="Masneuf Pomarede, Isabelle" sort="Masneuf Pomarede, Isabelle" uniqKey="Masneuf Pomarede I" first="Isabelle" last="Masneuf-Pomarede">Isabelle Masneuf-Pomarede</name><name sortKey="Masneuf Pomarede, Isabelle" sort="Masneuf Pomarede, Isabelle" uniqKey="Masneuf Pomarede I" first="Isabelle" last="Masneuf-Pomarede">Isabelle Masneuf-Pomarede</name><name sortKey="Miot Sertier, Cecile" sort="Miot Sertier, Cecile" uniqKey="Miot Sertier C" first="Cécile" last="Miot-Sertier">Cécile Miot-Sertier</name><name sortKey="Panfili, Aurelie" sort="Panfili, Aurelie" uniqKey="Panfili A" first="Aurélie" last="Panfili">Aurélie Panfili</name><name sortKey="Salin, Franck" sort="Salin, Franck" uniqKey="Salin F" first="Franck" last="Salin">Franck Salin</name></country><country name="Australie"><noRegion><name sortKey="Curtin, Chris" sort="Curtin, Chris" uniqKey="Curtin C" first="Chris" last="Curtin">Chris Curtin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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